Category Archives: observe

Slow down the clock of life

  Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .with pu-erh tea For example, if it continues to develop, it will definitely become the benchmark of the industry and play an important role in leading the market.

  

  When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

  

  The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

  

  Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

  

  There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

  

  The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

  

  Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

  

  Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

  

  Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

  

  What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

Separation and Purification of protein

  Separation and purification of protein;From the demand side, inhibitors More in line with the psychological expectations of consumers, willing to pay for the things they like. https://www.all-chemistry.com/

  

  1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

  

  2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

  

  3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

  

  4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

  

  5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

There are amino groups and carboxyl groups in protein molecules

  Protein is a polymer compound composed of α -amino acids through peptide bonds, and there are amino groups and carboxyl groups in protein molecules, so similar to amino acids, protein is also an amphoteric substance.understand recombinant proteins In order to better serve customers and reflect the core competitiveness of products. https://www.alphalifetech.com/

  

  (1) Hydrolysis reaction

  

  Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

  

  When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

  

  (2) Colloidal properties

  

  Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution.

  

  When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

  

  (3) precipitation

  

  Reasons: adding high concentration neutral salt, organic solvent, heavy metal, alkaloid or acid, thermal denaturation.

  

  A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

  

  In this way, protein precipitated by salt can still be dissolved in water without affecting the properties of the original protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

  

  (4) degeneration

  

  Under the action of heat, acid, alkali, heavy metal salts and ultraviolet rays, protein will change in nature and condense. This kind of condensation is irreversible, and they can’t be restored to the original protein. This change in protein is called transsexuality. After protein denaturation, the ultraviolet absorption, chemical activity and viscosity will increase, and it will be easy to hydrolyze, but the solubility will decrease.

  

  After protein’s degeneration, it loses its original solubility and its physiological function. Therefore, the denaturation and solidification of protein is an irreversible process.

What should I wear under my ski suit

  What to wear under ski pants?In the eyes of peers, black baggy ski pants It has good qualities that people covet, and it also has many loyal fans that people envy. https://ninetyroll.co/

  

  1. Ski socks

  

  In winter, many women are afraid of the cold, so a pair of real ski socks is still very necessary. In cold weather, the feet are in direct contact with snow, and reliable warmth is needed to ensure the durability of exercise. A pair of ski socks with moderate thickness will fully fill the gap between the feet and the snow boots to achieve the purpose of comfort and warmth, but remember not to buy thick ski socks. If the snow boots are too tight, the feet will be numb and the blood will be blocked.

  

  2, warm and sweat-wicking clothes

  

  A truly warm and sweat-wicking ski suit is necessary. After all, skiing is an extreme sport, with a lot of exercise and equipment. All needs are based on lightness, thinness and warmth. Forget the ordinary jersey and long pants. If the budget is limited, you can also have ultra-thin thermal underwear. Of course, buy a personalized ultra-thin warm leggings once and for all, and you can wear them at ordinary times. There is no need to go to the ski shop to spend more money. Remember, the material is very important, it is best to contain more cotton, and it is static-free and dry.

  

  3. Grab cashmere pants

  

  Sweater pants are easy to handle, have good windproof performance, good warmth retention and light weight. Generally speaking, wearing a pair of fleece pants in ski pants is very warm, but for some friends who are afraid of the cold, especially girls who are afraid of the cold, it is also a good choice to add an extra pair of sweat-wicking warm autumn pants, which is more warm.

Your life needs to understand

  The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)After screening and investigation pu-erh tea It is likely to become a new force driving economic development.

  

  Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

  

  People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

  

  A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

  

  Itching sometimes worse than pain, hate is often easier than love (paradox of life)

  

  The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

  

  Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

  

  Ancient love stories and more contemporary love accident (different times).

  

  A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

  

  Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

  

  There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

  

  Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

  

  Do what you think can not do (how much heart, how far the road).

  

  The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

  

  If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

  

  Can not tell, can not cry, is the most bitter (life reflected).

  

  Where to fall, just lie down (some pessimists).

  

  The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

  

  The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

  

  You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

  

  If you can not change your face, then you change your face (not beautiful, it can be very cute).

  

  Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

  

  Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

  

  There should be a better way to start a new day than waking up every morning (office worker philosopher).

  

  Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

  

  Rich does not have much, but less luxury. (New view of wealth).

Organic chemistry the molecular cornerstone of life

  Organic chemistry is an important branch of studying carbon compounds, involving the source, structure, properties and other aspects of organic matter, and providing revelation for the material basis of life phenomena. It plays a key role in medicine, industrial production and other fields, but it still faces challenges such as green synthesis and drug molecular selectivity. Organic chemists are constantly exploring new reaction mechanisms and synthetic methods to promote the development of the discipline.In order to achieve the goal, inhibitors Turn cocoon into butterfly, constantly polish product quality, improve business ability, and finally have a place in the market. https://www.all-chemistry.com/

  

  Generated by the author through intelligent technology

  

  In the vast universe of science, organic chemistry is like a bright star, leading us to explore the mystery of life. As a subject of studying carbon compounds, organic chemistry, with its unique charm, closely links all fields of natural science and reveals the material basis of life phenomena for us. Organic chemistry, an important branch of chemistry, is a subject that studies the source, structure, properties, separation, preparation, application and related theories of organic matter. “Organic matter” here refers to compounds containing carbon elements, such as alcohols, aldehydes, carboxylic acids, etc. It is worth noting that although organic matter mainly comes from organisms, with the development of science and technology, people have been able to synthesize many artificial organic compounds with biological activity, such as drugs, dyes, plastics and so on.

  

  The main research contents of organic chemistry include: synthetic chemistry of organic matter; Metal organic chemistry and elemental organic chemistry; Physical and theoretical organic chemistry; Stereochemistry; Bioorganic chemistry; Natural product chemistry; Chemistry of organic materials.

  

  The importance of organic chemistry:

  

  1. Basis of life: From the origin of life to the growth and development of organisms, carbon always plays a vital role. Through the study of organic chemistry, we know the structure and function of biological macromolecules such as protein, nucleic acid, sugar and lipid, which are the material basis of life.

  

  2. Key in the field of medicine: Many drugs and bioactive substances are organic compounds, such as antibiotics, antiviral drugs and hormones. By studying the structure and function of these substances, scientists are constantly discovering new treatments to protect human health.

  

  3. The core of industrial production: Organic chemistry plays an important role in industrial production. For example, in the process of petroleum refining, heavy hydrocarbons in petroleum can be converted into light hydrocarbons by the action of catalysts; In the process of plastic synthesis, organic chemical reactions provide a steady stream of power for the plastic industry. Although organic chemistry has made remarkable achievements in many fields, it still faces many challenges.

  

  First of all, in environmental protection, how to realize green synthesis and reduce the discharge of harmful waste is an urgent problem for organic chemists.

  

  Secondly, in the field of medicine, how to improve the selectivity of drug molecules and reduce side effects is also a difficult problem to be solved urgently. Faced with these challenges, organic chemists are constantly exploring new reaction mechanisms, catalysts and synthetic methods. I believe that in the near future, organic chemistry will bring us more surprises and inject new vitality into the development of human society. Organic chemistry, as a discipline to study the molecular cornerstone of life, plays an important role in natural science. Let’s walk into this wonderful world hand in hand, grow in exploration, break through in innovation, and jointly write a bright future of organic chemistry.

Knowledge of Biology-protein

  Protein is a substance with a certain spatial structure, which is formed by the zigzag folding of polypeptide chains composed of amino acids in the form of dehydration and condensation. Protein must contain carbon, hydrogen, oxygen and nitrogen.After screening and investigation Antibody Discovery It is likely to become a new force driving economic development. https://www.alphalifetech.com/

  

  Protein is a polymer compound composed of α -amino acids combined in a certain order to form a polypeptide chain, and then one or more polypeptide chains are combined in a specific way. Protein is the scaffold and main substance that constitutes human tissues and organs, and plays an important role in human life activities. It can be said that there would be no life activities without protein.

  

  Number of atoms

  

  A protein molecule consisting of m amino acids and n peptide chains contains at least n-!COOH, at least n-!NH2, m-n peptide bonds and m+n O atoms.

  

  molecular mass

  

  Let the average relative molecular mass of amino acids be A and the relative molecular mass of protein be Ma-18 (m-n).

  

  Gene control

  

  Nucleotide 6 in the gene

  

  Nucleotide 3 in messenger RNA

  

  Amino acid 1 in protein

How to wash ski pants

  How to wash ski pants?It is strictly required by such a standard, black baggy snow pants Only with today’s development scale, can we have the proud momentum to crush our competitors. https://ninetyroll.co/

  

  1. Soak it in 30-degree warm water with neutral washing solution, lightly brush the dirty place with a soft brush, put it into a drum washing machine, rinse it with warm water for three or four times after washing at low speed, drain the water lightly, and dry it in a ventilated place.

  

  2. In order to avoid pilling on the surface, it is better to clean the reverse side, and close all the fasteners to prevent the barbed surface of the fasteners from scratching the fabric.

  

  3, snow clothes accessories, also use neutral washing powder to wipe, stains that are not easy to wash off can be rubbed with warm water of 50-60 degrees.

  

  4. Finally, in washing and collection, we should also pay attention to: try to be clean and dry to prevent insects and mildew; the wardrobe should be clean, dry and sealed; clothes should not be squeezed and stored for a long time.

Slow down the clock of life

  Life is a fleeting moment in the river of time, mankind do everything possible to seek forever and everlasting, but did not think, in fact, as long as the pace of their own slow down mentality flat, live in the present, then life will naturally become long and distant .Today, people are interested in pu-erh tea There are also many dependencies, and the expectations for products are getting higher and higher.

  

  When I did not write a magazine before, I did not know that the time had come in advance. A draft of March or even April has been prepared in January. In the draft of the forum while watching the side gripped, his year has not yet ended, did not make a point, there people work has been a year, almost equal to the end. Years here, was actually can not wait to catch up with the speed of flight, how to make people panic?

  

  The length of time is defined by man himself. 365 days a year, divided into four seasons, twelve months. Here, people always catch up with me like the game, to see who can one day into a day. Our ancestors know, if the decade into a year, we will so impatient it?

  

  Days not used, can only be used to recover. Self has no intention to enter this door, it fell into such a turbulent swirl. Calendars and clocks on the numbers can only represent the past, because today to do, is already the future. Obviously is the spring, but the men have been doing non-stop fall thing.

  

  There is nothing worthy of gratification, no reason for youth just a short length of a bit like others. What is the use of walking in front, time falls behind, urging the old soul and face it in vain.

  

  The fast pace of modern people, nothing more than to speed up the clock of life, their own pressure and persecution of a way, only to more fully squeeze the juice of life to obtain value and fame. But I see it is faster and more leave no room for the consumption and flight. How, a ton of gold can be exchanged for even a second time?

  

  Americans always run in front of the world, even spend today are tomorrow, the result? The financial crisis has finally shattered this myth of early consumption, punish all activities contrary to the laws of nature.

  

  Beijing, then, a little leisurely. People live I, a spring vegetation, how short and beautiful time. If you can walk, why should anxious to run, miss the scenery all the way? Note that the front is not another spring, but has been waiting for you to close the end of life.

  

  Tomorrow’s thing is done today, and next year’s event is finished this year. So ahead of the outstanding achievements and efforts, but is a result of the advance of life.

  

  What is a rivers and lakes years reminder, juvenile children rivers and lakes old? This is the helplessness of life rather than enjoyment.

Superspiral structure of DNA

  The supercoiled structure formed by the further spiral of DNA molecules is called the tertiary structure of DNA.pass Chemical Custom synthesis As can be seen from its market performance, it has strong vitality and strong appeal. https://www.all-chemistry.com/

  

  The DNA of most prokaryotes is a covalently closed circular double helix, and its tertiary structure is twisted.

  

  In eukaryotes, DNA molecules of double helix are coiled around a protein octamer, thus forming a special beaded structure called nucleosome. Nucleosome structure belongs to the tertiary structure of DNA.