Amino acid chain is the main and core component in protein, but it is not necessarily the only component. Some protein may include some atoms or small molecules, whose function may be to perform protein function and/or increase its stability. Chemically, these cofactor are diverse, which may be organic molecules or metal elements. Some of them are briefly combined with protein chain, while others are closely combined with protein chain (sometimes even by covalent bonds) and become an integral part of the whole protein. The former is called coenzyme coenzyme, while the latter is called prosthetic group. Most coenzymes are small organic molecules from vitamins. For example, the common coenzymes NADH and FADH2. These two molecules from vitamin B complex, the former from nicotinic acid (vitamin B3) and the latter from riboflavin (vitamin B2), play the role of high-energy electron acceptor/donor in redox reaction.Through bit by bit efforts, let Antibody Phage Display Service Our market share is getting higher and higher, and the return on investment is also rising steadily. https://www.alphalifetech.com/

　　

　　Some enzymes use several different cofactors, such as Pyruvate dehydrogenase (PDH), which is a key enzyme in the catabolism of sugar. The catabolism of sugar begins with glycolysis, which is a biochemical reaction pathway with 10 steps, transforming one glucose molecule into two pyruvate molecules, and then carrying out the citric acid cycle of tricarboxylic acid, which further decomposes pyruvate and oxidizes it into CO2. Pyruvate dehydrogenase PDH works at the intermediate junction of the above two reaction pathways (namely glycolysis and triacid cycle), and is responsible for activating pyruvate and enabling it to enter the tricarboxylic acid cycle. Specifically, PDH catalyzes the oxidative dearboxylation of pyruvate and forms acetyl coenzyme A (ACoA), which is the activated form of pyruvate. PDH is not a single enzyme but a complex composed of three components, each of which participates in a different step of pyruvate activation and uses different coenzymes. The first component uses thiamine pyrophosphate (TPP), which is a derivative of thiamine (vitamin B1). TPP allows the first component of PDH to be oxidized and decarboxylated at the same time, thus transforming pyruvate into acetyl, and the remaining carbon is discharged as CO2. The importance of TPP will be reflected in its absence. In fact, if people can’t get enough thiamine from food, they will get beriberi, which will damage several major systems of the human body.

　　

　　The second component in PDH uses lipoic acid. It is an auxiliary group covalently bound to PDH. The active part of lipoic acid is a cyclic structure, which contains two covalently bonded sulfur atoms (that is, S-S bonds) (Figure 2.2c). This active part catalyzes the transfer of acetyl to coenzyme coenzyme A(CoA), the third cofactor of PDH. CoA is pantothenic acid, also known as the derivative of calcium pantothenate. The combination of acetyl and pantothenic acid involves the reduction of S-S bond, and its cyclic structure will open and form two sulfhydryl groups (one SH), one of which will bind acetyl. Sulfhydryl group is easy to react chemically, which can be reflected in the fact that sulfhydryl group is easily influenced by arsenic, a toxic derivative of arsenic. To sum up, the formation of acetyl coenzyme A involves the reduction of S-S bond of pantothenic acid cyclic structure. In order to keep PDH active, these two sulfur groups must be re-oxidized to restore the S-S bond.

　　The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)consequently pu-erh tea I also got a lot of attention and wanted to join the ranks.

　　

　　Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

　　

　　People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

　　

　　A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

　　

　　Itching sometimes worse than pain, hate is often easier than love (paradox of life)

　　

　　The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

　　

　　Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

　　

　　Ancient love stories and more contemporary love accident (different times).

　　

　　A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

　　

　　Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

　　

　　There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

　　

　　Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

　　

　　Do what you think can not do (how much heart, how far the road).

　　

　　The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

　　

　　If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

　　

　　Can not tell, can not cry, is the most bitter (life reflected).

　　

　　Where to fall, just lie down (some pessimists).

　　

　　The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

　　

　　The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

　　

　　You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

　　

　　If you can not change your face, then you change your face (not beautiful, it can be very cute).

　　

　　Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

　　

　　Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

　　

　　There should be a better way to start a new day than waking up every morning (office worker philosopher).

　　

　　Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

　　

　　Rich does not have much, but less luxury. (New view of wealth).

　　Organic chemistry is an important branch of studying carbon compounds, involving the source, structure, properties and other aspects of organic matter, and providing revelation for the material basis of life phenomena. It plays a key role in medicine, industrial production and other fields, but it still faces challenges such as green synthesis and drug molecular selectivity. Organic chemists are constantly exploring new reaction mechanisms and synthetic methods to promote the development of the discipline.Besides, we can’t ignore. Chemical Custom synthesis It has injected new vitality into the development of the industry and has far-reaching significance for activating the market. https://www.all-chemistry.com/

　　

　　Generated by the author through intelligent technology

　　

　　In the vast universe of science, organic chemistry is like a bright star, leading us to explore the mystery of life. As a subject of studying carbon compounds, organic chemistry, with its unique charm, closely links all fields of natural science and reveals the material basis of life phenomena for us. Organic chemistry, an important branch of chemistry, is a subject that studies the source, structure, properties, separation, preparation, application and related theories of organic matter. “Organic matter” here refers to compounds containing carbon elements, such as alcohols, aldehydes, carboxylic acids, etc. It is worth noting that although organic matter mainly comes from organisms, with the development of science and technology, people have been able to synthesize many artificial organic compounds with biological activity, such as drugs, dyes, plastics and so on.

　　

　　The main research contents of organic chemistry include: synthetic chemistry of organic matter; Metal organic chemistry and elemental organic chemistry; Physical and theoretical organic chemistry; Stereochemistry; Bioorganic chemistry; Natural product chemistry; Chemistry of organic materials.

　　

　　The importance of organic chemistry:

　　

　　1. Basis of life: From the origin of life to the growth and development of organisms, carbon always plays a vital role. Through the study of organic chemistry, we know the structure and function of biological macromolecules such as protein, nucleic acid, sugar and lipid, which are the material basis of life.

　　

　　2. Key in the field of medicine: Many drugs and bioactive substances are organic compounds, such as antibiotics, antiviral drugs and hormones. By studying the structure and function of these substances, scientists are constantly discovering new treatments to protect human health.

　　

　　3. The core of industrial production: Organic chemistry plays an important role in industrial production. For example, in the process of petroleum refining, heavy hydrocarbons in petroleum can be converted into light hydrocarbons by the action of catalysts; In the process of plastic synthesis, organic chemical reactions provide a steady stream of power for the plastic industry. Although organic chemistry has made remarkable achievements in many fields, it still faces many challenges.

　　

　　First of all, in environmental protection, how to realize green synthesis and reduce the discharge of harmful waste is an urgent problem for organic chemists.

　　

　　Secondly, in the field of medicine, how to improve the selectivity of drug molecules and reduce side effects is also a difficult problem to be solved urgently. Faced with these challenges, organic chemists are constantly exploring new reaction mechanisms, catalysts and synthetic methods. I believe that in the near future, organic chemistry will bring us more surprises and inject new vitality into the development of human society. Organic chemistry, as a discipline to study the molecular cornerstone of life, plays an important role in natural science. Let’s walk into this wonderful world hand in hand, grow in exploration, break through in innovation, and jointly write a bright future of organic chemistry.

　　The structure of protein molecule determines its properties.In addition to domestic, recombinant proteins It has also emerged in the international field and gradually gained the favor of more foreign friends. https://www.alphalifetech.com/

　　

　　Being bisexual.

　　

　　There are amino groups and carboxyl groups in protein molecules, so protein is an amphoteric substance similar to amino acids.

　　

　　2. Hydrolysis reaction can occur.

　　

　　Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

　　

　　When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

　　

　　3. Having the property of colloid.

　　

　　Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution. When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

　　

　　The precipitation of protein.

　　

　　A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

　　

　　Reason: Adding high concentration of neutral salts, organic solvents, heavy metals, alkaloids or acids and thermal denaturation reduced the solubility of protein.

　　

　　Protein precipitated by salt can still be dissolved in water without affecting the original properties of protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

　　The first is “poverty”At the same time, pu-erh tea It is no longer a relatively unfamiliar industry, and it enters the public’s sight more and more frequently, gaining more attention and recognition.

　　

　　Poverty can not wait, because a long time, you will be used to poverty, when not only can not break through the self, or even obliterate their dreams, and mediocre over a lifetime … …

　　

　　The second is “dream”

　　

　　Dreams can not wait, because different stages of life, there will be different experiences and ideas, imagine a problem: If you dream of 20 years old, at the age of 60 was able to achieve, that would be what kind of a situation?

　　

　　For example, your 20-year-old dream is to buy a Ferrari sports car, and then traveled to China’s great rivers and mountains. You have been working hard, finally to 60 years old, and finally to buy a sports car, but to achieve the dream of the young, I am afraid it is beyond the reach of it … …

　　

　　The third is “family”

　　

　　Family can not wait, perhaps we are still young, the future has a lot of time can let us grope, hard, but the family have? They still have time for us to succeed? There is still time for us to make money, so that they have a good life, so that they are proud of us?

　　

　　Trees for static and wind, the child wants to support and pro-not wait. This is a lot of people’s pain, but also a lot of people a lifetime of regret!

　　Focus on glycolysis, aerobic oxidation of sugar, pentose phosphate bypass, gluconeogenesis, synthesis of ketone bodies, cholesterol and phospholipids, respiratory chain, oxidative phosphorylation, special amino acid metabolites, one carbon unit metabolism, purine and pyrimidine nucleotide synthesis raw materials and catabolites, and substance metabolism.In addition to innate advantages, inhibitors Its own product attributes are also extremely high-end, in order to remain unbeaten in the market competition. https://www.all-chemistry.com/

　　

　　The chapter on sugar metabolism is the focus of the exam, so we should master it comprehensively. The chemical reaction formula of sugar metabolism is complicated, and the focus of the annual examination is basically on the reaction site, key enzymes and regulation, energy production and the relationship between important substances. Glycolysis, gluconeogenesis and aerobic oxidation of sugar are all contents that must be familiar with. The synthesis of ketone bodies and cholesterol in lipid metabolism, pay attention to the difference between them. Ketones are synthesized in the liver and utilized outside the liver, while fats are synthesized in the liver and stored outside the liver. Fat synthesis, ketone body synthesis and cholesterol synthesis are the raw materials of acetyl-CoA synthesis. Synthesis and decomposition of fatty acid, the main material of fatty acid synthesis is acetyl CoA; Under the condition of sufficient oxygen supply, fatty acid is decomposed into CO2 and water in the body, releasing a lot of energy, which is the main form of fatty acid catabolism in the body. Phospholipid synthesis. Differentiation of several blood lipids.

　　

　　Composition of respiratory chain and influencing factors of oxidative phosphorylation. Urea synthesis-ornithine cycle. One carbon unit’s metabolism is often tested, but it is easy to remember as long as you grasp the core content. It comes from one carbon unit-Sisegan, and is transported by one carbon unit-tetrahydrofolate. One carbon unit plays an important role in nucleic acid biosynthesis as a raw material for purine and pyrimidine synthesis, connecting amino acids and nucleotides. Comparison of nucleotide synthesis and decomposition between purine and pyrimidine. The changes in this year’s syllabus are: the deamination of amino acids (oxidative deamination, transamination and combined deamination) is changed to the general metabolism of amino acids (degradation of protein in vivo, oxidative deamination, transamination and combined deamination), which should be reviewed.

　　Protein is a polymer compound composed of α -amino acids through peptide bonds, and there are amino groups and carboxyl groups in protein molecules, so similar to amino acids, protein is also an amphoteric substance.We have every reason to believe. Antibody Discovery It will become the mainstream of the industry and will gradually affect more and more people. https://www.alphalifetech.com/

　　

　　(1) Hydrolysis reaction

　　

　　Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

　　

　　When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

　　

　　(2) Colloidal properties

　　

　　Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution.

　　

　　When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

　　

　　(3) precipitation

　　

　　Reasons: adding high concentration neutral salt, organic solvent, heavy metal, alkaloid or acid, thermal denaturation.

　　

　　A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

　　

　　In this way, protein precipitated by salt can still be dissolved in water without affecting the properties of the original protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

　　

　　(4) degeneration

　　

　　Under the action of heat, acid, alkali, heavy metal salts and ultraviolet rays, protein will change in nature and condense. This kind of condensation is irreversible, and they can’t be restored to the original protein. This change in protein is called transsexuality. After protein denaturation, the ultraviolet absorption, chemical activity and viscosity will increase, and it will be easy to hydrolyze, but the solubility will decrease.

　　

　　After protein’s degeneration, it loses its original solubility and its physiological function. Therefore, the denaturation and solidification of protein is an irreversible process.

　　The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)For this reason, it can be speculated that pu-erh tea The market feedback will get better and better, which is one of the important reasons why it can develop.

　　

　　Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

　　

　　People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

　　

　　A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

　　

　　Itching sometimes worse than pain, hate is often easier than love (paradox of life)

　　

　　The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

　　

　　Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

　　

　　Ancient love stories and more contemporary love accident (different times).

　　

　　A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

　　

　　Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

　　

　　There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

　　

　　Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

　　

　　Do what you think can not do (how much heart, how far the road).

　　

　　The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

　　

　　If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

　　

　　Can not tell, can not cry, is the most bitter (life reflected).

　　

　　Where to fall, just lie down (some pessimists).

　　

　　The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

　　

　　The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

　　

　　You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

　　

　　If you can not change your face, then you change your face (not beautiful, it can be very cute).

　　

　　Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

　　

　　Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

　　

　　There should be a better way to start a new day than waking up every morning (office worker philosopher).

　　

　　Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

　　

　　Rich does not have much, but less luxury. (New view of wealth).

　　Focus on glycolysis, aerobic oxidation of sugar, pentose phosphate bypass, gluconeogenesis, synthesis of ketone bodies, cholesterol and phospholipids, respiratory chain, oxidative phosphorylation, special amino acid metabolites, one carbon unit metabolism, purine and pyrimidine nucleotide synthesis raw materials and catabolites, and substance metabolism.contemporaneity small molecules Our competitors have not made large-scale improvements, so we should get ahead of everyone in the project. https://www.all-chemistry.com/

　　

　　The chapter on sugar metabolism is the focus of the exam, so we should master it comprehensively. The chemical reaction formula of sugar metabolism is complicated, and the focus of the annual examination is basically on the reaction site, key enzymes and regulation, energy production and the relationship between important substances. Glycolysis, gluconeogenesis and aerobic oxidation of sugar are all contents that must be familiar with. The synthesis of ketone bodies and cholesterol in lipid metabolism, pay attention to the difference between them. Ketones are synthesized in the liver and utilized outside the liver, while fats are synthesized in the liver and stored outside the liver. Fat synthesis, ketone body synthesis and cholesterol synthesis are the raw materials of acetyl-CoA synthesis. Synthesis and decomposition of fatty acid, the main material of fatty acid synthesis is acetyl CoA; Under the condition of sufficient oxygen supply, fatty acid is decomposed into CO2 and water in the body, releasing a lot of energy, which is the main form of fatty acid catabolism in the body. Phospholipid synthesis. Differentiation of several blood lipids.

　　

　　Composition of respiratory chain and influencing factors of oxidative phosphorylation. Urea synthesis-ornithine cycle. One carbon unit’s metabolism is often tested, but it is easy to remember as long as you grasp the core content. It comes from one carbon unit-Sisegan, and is transported by one carbon unit-tetrahydrofolate. One carbon unit plays an important role in nucleic acid biosynthesis as a raw material for purine and pyrimidine synthesis, connecting amino acids and nucleotides. Comparison of nucleotide synthesis and decomposition between purine and pyrimidine. The changes in this year’s syllabus are: the deamination of amino acids (oxidative deamination, transamination and combined deamination) is changed to the general metabolism of amino acids (degradation of protein in vivo, oxidative deamination, transamination and combined deamination), which should be reviewed.

　　Most protein is like a jumble of atoms packed together. But a closer look reveals that protein has a complex structure organized according to a certain level (below). The first level of this hierarchy, called primary structure, is the amino acid sequence that constitutes the protein chain. Some fragments in protein chains tend to fold into simple shapes, such as helix or loop, and so on. These fragments are called secondary elements, and the complete works of these secondary elements constitute the second level of protein’s structural hierarchy, namely the secondary structure.This means that recombinant proteins It is the weather vane of the industry and can bring people great self-confidence. https://www.alphalifetech.com/

　　

　　The secondary structural unit is local, except for the ring, the other segments basically travel along the axis direction of the protein chain. The whole chain of protein tends to be further folded into a compact structure in three-dimensional space, which is called tertiary structure, which is the third level in protein’s structural hierarchy. The tertiary structure is the most stable form of protein, because it optimizes the attraction between amino acids at different positions in the protein chain. Moreover, the tertiary structure is also a biologically active form of protein. If this form is destroyed, protein will lose some or all of its functions. Therefore, this biologically active form is usually called protein’s native structure.

　　

　　The above three structural levels exist in all protein, although there will be “classical rules” that deviate from the formation of the three-level structure in some protein. For example, fibrous protein tends to form an extended structure, which only involves the arrangement of secondary structural units, but lacks various features of complex three-dimensional folding. Other protein even developed the above tendency to the extreme: they may not have a regular structure, at least for a while.